Genetic population structure of Brown Boobies largely matches patterns of morphological variation (Steeves et al. 2003, Morris-Pocock et al. 2010, 2011). Mitochondrial haplotypes were not shared between the eastern and central Pacific or between the eastern Pacific and Caribbean (Steeves et al. 2003), and colonies grouped into four major, genetically differentiated populations; Caribbean, central Atlantic, Indo-central Pacific, and eastern Pacific (Morris-Pocock et al. 2011). The eastern Pacific population was found to be the most different genetically and was estimated to have diverged from all other populations approximately one million years ago (Morris-Pocock et al. 2011). These populations have diverged because of a combination of physical barriers (the Isthmus of Panama and the Eastern Pacific Basin) and a behavioral tendency in the Brown Booby to forage closer to shore than other booby species (Steeves et al. 2003).